Skip to main content
. 2019 Jun 24;14(6):e0218854. doi: 10.1371/journal.pone.0218854

Fig 7. CC-NHDFs release soluble signals that accelerate aggregation and coalescence.

Fig 7

A. The transmembrane preparation employed to assess the presence of soluble factors. B. Representative images of aggregation of MDA-MB-231 cells after 2 and 4 days in a preparation with F-NHDFs or CC-NHDFs in the upper insert. C. Gel zymogram and quantitation of pixel intensities of MMPs in FGM directly from the bottle of sterile, unused media (no cells); F-FGM, media from 72 hour cultures of F-NHDFs; CC-FGM/MB-231, media from 72 hour MDA-MB-231 cultures; and CC-FGM/CC-NHDF, media from 72 hour CC-NHDF. D. Aggregation after 48 hours of MDA-MB-231 preparations over a CC-NHDF substratum in the absence and presence of the MMP inhibitor GM6001. E. Western blot of media probed with anti-SDF-1α/CXCL12 mAb. Recombinant SDF-1α/CXCL12 was used as a positive control. F. Coalescence after 48 and 72 hours of untreated MDA-MB-231, MDA-MB-231 cells treated with SDF-1α/CXCL12, MDA-MB-231 cells treated with SDF-1α/CXCL12 plus anti-SDF-1α/CXCL12 mAb, and MDA-MB-231 cells treated with rIL-6.