Table 1. Primer sequences of genes used for validation study using qRT-PCR.

S. No.	Chro. No.	Gene ID	Forward Primer	Reverse primer	Amplicon length (bp)	Gene Name / Functional description from Cotton FGD* [28]
1	A05	Gh_A05G3682	GTTGGGGCTTCGAAGGGTAA	AACGTTGCTTGAGGAGTGGT	133	Beta-galactosidase 16
2	D11	Gh_D11G3302	AAGATCCCCGGCGTTTACTC	ATGGGGGACTTGGGAGTGTA	118	Expansin A4
3	D04	Gh_D04G1783	AAAGGCTACGGCAATGGACA	TGCCTCCACCTTACATGCAG	129	Pectin lyase-like superfamily protein
4	A01	Gh_A01G0900	GGATGGCATCGACTTTTGTGG	AATGCAAAAACGCCTTGCGA	105	Tubulin beta-1 chain
5	D07	Gh_D07G0486	GTTGCCCTAGTGAGTGCGAT	ATCCGAGCTTTCTTGGGAGC	149	Homolog of Medicago truncatula MTN3
6	A08	Gh_A08G0788	TCGATTTCTACAGTACCAGCAG	TTATGACGGCTGCCCTTCTC	109	Seven transmembrane MLO family protein
7	A01	Gh_A01G1209	CCAAATGATTGGCCTTGCCC	CACCACCCTCATTGTGACCA	103	TBP-associated factor 15
8	D11	Gh_D11G3072	CCCGAGAACACTACCAACGAC	CGGTTTCCCTTCAACAGCCT	150	Acyl-CoA-binding protein 6
9	A05	Gh_A05G0419	GGCAATGGCCATCTTCACAC	ACTAGGCCCGTCTTCATCCT	145	Reticulon family protein
10	D01	Gh_D01G1631	CCAGCAAACCCAACCAGAGT	AGTAGTGATGGTGCTGCAAGT	109	myb domain protein 4
11	A04	Gh_A04G1346	AAAACGCACGTCACCTACCT	TCCGGTACTGTTTGCTTCGG	10	DUF828 Plant protein with unknown function
12	A11	Gh_A11G0133	ATCCCTCCTCTCGTCGGAAA	GGTTGCCCGCATTGTTCTTT	111	RNA-binding family protein
13	A01	Gh_A01G0153	CCGGAGCTTCCGAGGTATTC	CTCGACGGAACCGAAAAGGA	129	Jasmonate-zim-domain protein 10
14	A09	Gh_A09G0200	AAGACGTTCTCACGGCCATT	TTCCTCCATCCAGCAAAGCC	144	Inositol monophosphatase family protein
15	D04	Internal Control	GCGATCTGGTAAGGAGCTTG	GGAGAAGGTTTCCACAACCA	106	Gossypium hirsutum EF1A1

* Cotton Functional Genomics Database (CottonFGD) is a validated integrates transcriptomic database for analysis platform for cotton (Gossypium spp).