Table 2. Quality screening and mapping statistics of RNA-Seq data.
Reads | JS178 (CGMS) | JB178 (Maintainer) | JR178 (Restorer) |
---|---|---|---|
No. of reads generated | 53,048,502 | 61,704,635 | 67,960,025 |
No. of reads passed quality trimming | 48,457,321 | 55,645,046 | 61,579,735 |
No. of reads mapped to reference genome of Cotton (Gossypium hirsutum L.) | 34,037,349 (70.24%) | 39,800,204 (71.52%) | 45,395,238 (73.71%) |
Total no. of bases mapped | 5,325,913,900 | 6,227,644,626 | 7,103,116,022 |
GC% of mapped reads | 43.17 | 43.24 | 43.26 |
Q25% of mapped reads | 94.23 | 94.19 | 94.26 |
Error% of mapped reads | 0.0128 | 0.0114 | 0.0112 |
Average length of mapped reads (bp) | 237 | 234 | 245 |