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. 2019 Jun 11;8:e46084. doi: 10.7554/eLife.46084

Figure 8. Cholesterol-TRPA1 interactions are essential for normal channel distribution in the membrane.

Figure 8.

(A–B) Confocal microscopy images of HEK293T cells expressing mTRPA1-mCherry (red) in the control condition (A) and after treatment with MCD (10 mM) (B). Expression pattern of mutant L850A TRPA1-mCherry (C and D) or Y852A TRPA1-mCherry (E and F). Cells were stained with the membrane dye CellBrite (green) and DAPI (blue). Scale bar, 5 µm. Right panels, 3D graph representing total membrane mCherry intensity corresponding the green-stained cell membrane area. (G) Mean membrane mCherry intensity in HEK293T expressing WT or mutated TRPA1 in control condition and after MCD treatment quantified from confocal microscopy images (n ≥ 5). (H) Mean mCherry membrane fractions quantified using custom design software representing distribution pattern of HEK293T expressing WT or mutated TRPA1 in control or after MCD treatment (n ≥ 5). *, p<0.05; **, p<0.01; #, p<0.05; ##, p<0.01; two-tailed Mann-Whitney U test.

Figure 8—source data 1. Intensities obtained from confocal microscopy and statistical analysis.
elife-46084-fig8-data1.xlsx (15.1KB, xlsx)
DOI: 10.7554/eLife.46084.024