Figure 2.

Appearance and Localization of AGM Myeloid Cells

(A and B) Flow cytometric analysis of E8.5 to E11.5 MacGreen (A) AGM and (B) YS cells. Percentage of GFP⁺ cells (top), CD45⁺ cells in GFP⁺ fraction (middle), and CD11b⁺F4/80⁺ cells in CD45⁺ fraction (bottom). Mean + SEM (n = 3/time, 2–4 AGM, 2–3 YS). ND, not done. One-way Anova with Bonferroni correction.

(C) Maximum projection confocal image of whole-mount E10 MacGreen embryo showing the trunk. A, aorta; V, vitelline artery. CD31⁺ endothelium, gray; macrophages, green; c-Kit⁺ IAHCs, magenta. Scale bar, 200 μm. High-magnification images of IAHC (right). Scale bars, 20 μm.

(D) Immunofluorescence of E10.5 MacGreen embryo transverse cryosection (10 μm) through aorta. AGM, aorta-gonad-mesonephros; NT, neural tube; S, somites. Yellow line, spatial boundaries of AGM. CD34⁺ endothelium is in magenta, macrophages in green, and nuclei in blue (DAPI). Scale bar, 100 μm.

(E) Total number of macrophages per μm² in non-hematopoietic tissues of trunk (non-AGM) and AGM region. n = 40 (10 random sections, 4 embryos). The data are represented as mean + SEM. One-way Anova with Dunnett post-hoc test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See Figures S3 and S4.