Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Candida albicans) | SC5314 | Hirakawa et al., 2015 (doi:10.1101/gr.174623.114) | RRID:SCR_013437 | |
| Strain, strain background (C. albicans) | P78042 | Hirakawa et al., 2015 (doi:10.1101/gr.174623.114) | ||
| Strain, strain background (C. albicans) | AMS3743 | This Study | In vitro evolution of P78042 in 128 ug/ml FLC for 100 generations | |
| Strain, strain background (C. albicans) | AMS3743_10 | This Study | In vitro evolution of AMS3743 in rich medium for 300 generations | |
| Strain, strain background (C. albicans) | AMS3743_10_S6 | This Study | Single colony from AMS3743_10 | |
| Antibody | Anti-Digoxigenin-AP Fab Fragments | Roche | 11093274910 RRID:AB_2734716 | (1:5000) |
| Sequenced-based reagent | PCR Primers | This Study | Supplementary file 7 | |
| Commercial assay or kit | Illumina Nextera XT Library Prep Kit | Illumina | 105032350 | |
| Commercial assay or kit | Illumina Nextera XT Index Kit | Illumina | 105055294 | |
| Commercial assay or kit | Illumina MiSeq v2 Reagent Kit | Illumina | 15033625 | 2 × 250 cycles |
| Commercial assay or kit | Blue Pippin 1.5% agarose gel dye-free cassette | Sage Science | 250 bp - 1.5 kb DNA size range collections, Marker R2 | Target of 900 bp |
| Commercial assay or kit | Qubit dsDNA HS kit | Life Technologies | Q32854 | |
| Commercial assay or kit | PCR DIG Probe Synthesis Kit | Roche | 11636090910 | |
| Commercial assay or kit | Agilent 2100 Bioanalyzer High Sensitivity DNA Reagents | Agilent Technologies | 5067–4626 | |
| Chemical compound, drug | Fluconazole (FLC) | Alfa Aesar | J62015 | |
| Software, algorithm | MUMmer Sutie | Kurtz et al., 2004 (doi:10.1186/gb-2004-5-2-r12) | v3.0 RRID:SCR_001200 | |
| Software, algorithm | Trimmomatic | Bolger et al., 2014 (doi:10.1093/bioinformatics/btu170) | v0.33 RRID:SCR_011848 | |
| Software, algorithm | BWA |
Li, 2013
(doi:10.1093/bioinformatics/btp324) |
v0.7.12 RRID:SCR_010910 | |
| Software, algorithm | Samtools | Li et al., 2009 (doi:10.1093/bioinformatics/btp352) | v0.1.19 RRID:SCR_002105 | |
| Software, algorithm | Genome Analysis Toolkit | McKenna et al., 2010 (doi:10.1101/gr.107524.110) | v3.4–46 RRID:SCR_001876 | |
| Software, algorithm | REPuter | Kurtz et al., 2001 (doi:10.1093/nar/29.22.4633) | V1.0 https://bibiserv.cebitec.uni-bielefeld.de/reputer | |
| Software, algorithm | Yeast Analysis Mapping Pipeline | Abbey et al., 2014 (doi:10.1186/s13073-014-0100-8) | v1.0 | |
| Software, algorithm | Graphpad Prism | https://www.graphpad.com | v6.0 RRID:SCR_002798 | |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/? | v2.0.0-rc-30/1.49 s RRID:SCR_003070 | |
| Software, algorithm | Integrative Genomics Viewer | Thorvaldsdóttir et al., 2013 (doi:10.1093/bib/bbs017) | v2.3.92 RRID:SCR_011793 | |
| Software, algorithm | R | https://www.r-project.org | v3.5.2 RRID:SCR_001905 | |
| Software, algorithm | Candida Genome Database | http://Candidagenome.org | RRID:SCR_002036 | |
| Other | Propidium Iodide | Invitrogen | P3566 | 25 ug/ml final concentration |
| Other | Ribonuclease A | MP Biomedicals | 101076 | 0.5 mg/ml final concentration |