Video 11. Impaired actin and BCR-Ag microcluster dynamics in CK-666-treated B cells interacting with APCs.

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DOI: 10.7554/eLife.44574.025

A20 D1.3 B cells expressing F-Tractin-GFP (green) were pre-treated for 1 hr with 100 µM CK-666 and then added to COS-7 APCs expressing mHEL-HaloTag (red). The B cell-APC contact site was imaged using ISIM. Images taken every 6.6 s. Video playback is 10 frames per second (66X real speed). See also Figure 4 and Figure 5.