Key resources table.
| Reagent type | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Sequence-based reagent | ON-TARGETplus Non-Targeting Pool, | Dharmacon | #D-001810-01-05 | |
| Sequence-based reagent | SMARTpool ON-TARGETplus, Dharmacon, | Dharmacon | #L-046642-01-0005 | |
| Other | RPMI-1640 without phenol red | Life Technologies | #32404014 | |
| Antibody | Goat anti-mouse IgG | Jackson ImmunoResearch | #115-005-008 | Coverslip coating 2.5 µg/cm2 |
| Antibody | Goat anti-mouse IgM | Jackson ImmunoResearch | #115-005-020 | Coverslip coating 2.5 µg/cm2 |
| Antibody | Rabbit anti-pZap70(Y319)/pSyk(Y352) | Cell Signaling Technologies | #2701 | Immunofluorescence 1:200 |
| Antibody | Rabbit anti-pCD79a(Y182) | Cell Signaling Technologies | #5173 | Immunofluorescence 1:400, Western blot 1:1000 |
| Antibody | Rabbit anti-CD79a | Gold et al., 1991 | Western blot 1:5000 | |
| Antibody | Rabbit anti-pCD19(Y531) | Cell Signaling Technologies | #3571 | Immunofluorescence 1:200, Western blot 1:1000 |
| Antiboday | Rabbit anti-CD19 | Cell Signaling Technologies | #3574 | Western blot 1:1000 |
| Antibody | Alexa Fluor 488 goat anti-rabbit IgG | Invitrogen | #A-11008 | Immunofluorescence 1:400 |
| Antibody | Alexa Fluor 647 goat anti-rat IgG | Invitrogen | #A-21247 | Immunofluorescence 1:400 |
| Antibody | Goat anti-mouse Ig kappa | Southern Biotech | #1050–01 | Stimulation 20 µg/ml |
| Antibody | Rabbit anti-ACTR3 (Arp3) | Santa Cruz | #sc-15390 | Western blot 1:1000 |
| Antibody | Rabbit anti-Arp2 | Abcam | #ab128934 | Western blot 1:1000 |
| Antibody | Mouse anti-β-actin | Santa Cruz | #sc-47778 | Western blot 1:5000 |
| Antibody | Rabbit anti-p34-Arc/ARPC2 | Millipore | #07–227 | Western blot 1:1000 |
| Antibody | Rabbit anti-pERK | Cell Signaling Technologies | #9101 | Western blot 1:1000 |
| Antibody | Rabbit anti-ERK | Cell Signaling Technologies | #9102 | Western blot 1:1000 |
| Antibody | Rabbit anti-pAkt | Cell Signaling Technologies | #9271 | Western blot 1:1000 |
| Antibody | Rabbit anti-Akt | Cell Signaling Technologies | #9272 | Western blot 1:1000 |
| Antibody | Goat anti-rabbit IgG(H + L)-HRP conjugate | Bio-Rad | #170–6515 | Western blot 1:3000 |
| Antibody | Goat anti-mouse IgM Fab fragment-Cy3-conjugated | Jackson ImmunoResearch | #115-167-020 | Single particle tracking 1 ng/ml |
| Antibody | Rat anti-mouse IgD Fab fragment-Cy3-conjugated | ATCC | #HB-250 (11–26 c) | Fab made by AbLab; SPT 100 ng/ml |
| Antibody | Rat anti-mouse CD19 Fab fragment-Cy3-conjugated | ATCC | #HB-305 (1D3) | Fab made by AbLab; SPT 10 ng/ml |
| Antibody | Rabbit anti-MHC II monoclonal antibody (M5/114) | Millipore | #MABF33 | Coverslip coating 0.25 µg/cm2 |
| Antibody | 2.4G2 monoclonal antibody | ATCC | #HB-197 | 0.25 µg/ml |
| Antibody | Anti-CD69-FITC | eBioscience | #11-0691-82 | Flow cytometry 1:200 |
| Antibody | Anti-CD86-APC | eBioscience | #17-0862-82 | Flow cytometry 1:200 |
| Biological sample (bovine) | Fibronectin | Sigma-Aldrich | #F4759 | Coverslip coating 5 µg/ml |
| Cell line (C. aethiops) | COS-7 | American Type Culture Collection | #CRL-1651; RRID:CVCL_0224 | |
| Cell line (M. musculus) | A20 | American Type Culture Collection | #TIB-208; RRID:CVCL_1940 | |
| Cell line (M. musculus) | A20 D1.3 | Batista and Neuberger, 1998 | F Batista (Ragon Institute, Cambridge, MA) | |
| Cell line (M. musculus) | B16F1 | American Type Culture Collection | #CRL-6323; RRID:CVCL_0158 | |
| Chemical compound, drug | CK-666 | Calbiochem | #CAS 442633-00-3 | 100 µM |
| Chemical compound, drug | CK-689 | Calbiochem | #CAS 170930-46-8 | 100 µM |
| Chemical compound, drug | (S)-nitro-blebbistatin (pnBB) | Cayman Chemicals | #CAS 856925-71-8 | 50 µM |
| Chemical compound, drug | HaloTag Janelia Fluor 549 ligand | Promega | #GA1110 | Cell labeling 1:2000 |
| Chemical compound, drug | HaloTag tetramethylrhodamine ligand | Promega | #G8251 | Cell labeling 1:2000 |
| Chemical compound, drug | Rhodamine phalloidin | Thermo Fisher | #R415 | Immunofluorescence 1:1000 |
| Chemical compound, drug | Alexa Fluor 568-conjugated phalloidin | Thermo Fisher | #A12380 | Immunofluorescence 1:1000 |
| Chemical compound, drug | Alexa Fluor 532-conjugated-phalloidin | Thermo Fisher | #A22282 | Immunofluorescence 1:1000 |
| Chemical compound, drug | ProLong Diamond anti-fade reagent | Thermo Fisher | #P36965 | |
| Chemical compound, drug | ECL detection reagent | GE Life Sciences | #RPN2106 | |
| Chemical compound, drug | CFSE | Invitrogen | #C1157 | Flow cytometry 2 µM |
| Chemical compound, drug | 7-AAD | Thermo Fisher | #A1310 | Flow cytometry 1:1000 |
| Commercial assay or kit | B cell isolation kit | Stemcell Technologies | #19854A | |
| Commercial assay or kit | AMAXA nucleofector kit V | Lonza | #VCA-1003 | |
| Commercial assay or kit | Ingenio electroporation solution | Mirus | #MIR 50117 | |
| Commercial assay or kit | Lipofectamine 3000 | Thermo-Fisher | #L3000008 | |
| Commercial assay or kit | Fab preparation kit | Pierce | #44985 | |
| Genetic reagent (M. musculus) | C57BL/6J | Jackson Laboratories | Stock #000664; MGI:3028467 | |
| Genetic reagent (M. musculus) | C57BL/6-Tg(IghelMD4)4Ccg/J | Jackson Laboratories | Stock #002595; MGI:2384162 | Goodnow et al., 1988 |
| Genetic reagent (M. musculus) | C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog | Moran et al., 2011 | MGI:5007644 | PMID:21606508; K Hogquist (University of Minnesota) |
| Peptide, recombinant protein | BAFF | R&D Systems | #8876-BF-010 | 5 ng/ml |
| Peptide, recombinant protein | IL-4 | R&D Systems | #404 ML-010 | 5 ng/ml |
| Recombinant DNA reagent | F-tractin-GFP | Johnson and Schell, 2009 | ||
| Recombinant DNA reagent | Myosin IIA-GFP | Addgene | #38297 | |
| Recombinant DNA reagent | mHEL-GFP | Batista et al., 2001 | F Batista (Ragon Institute, Cambridge, MA) | |
| Recombinant DNA reagent | mHEL-HaloTag | PMID: 29388105 | M Gold (University of British Columbia) | |
| Recombinant DNA reagent | Single-chain anti-mouse Ig kappa antibody | Ait-Azzouzene et al., 2005 | D Nemazee (Scripps Reseach Institute) | |
| Recombinant DNA reagent | β-Actin-GFP | R Nabi (University of British Columbia) | ||
| Software, algorithm | ISIM deconvolution | PMID: 26210400 | ||
| Software, algorithm | Softworx | Applided Precision Ltd.; GE Healthcare Life Sciences | ||
| Software, algorithm | FIJI | Schindelin et al., 2012 | https://imagej.net/Fiji | |
| Software, algorithm | MATLAB | MathWorks | R2015b | |
| Software, algorithm | MetaMorph | Molecular Devices | ||
| Software, algorithm | ICY | de Chaumont et al., 2012 | http://icy.bioimageanalysis.org | |
| Software, algorithm | FlowJo | Tree Star | v10.5.3 | |
| Software, algorithm | Robust Regression and Outlier Removal (ROUT) | Graphad | v7 | Motulsky and Brown, 2006 |