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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Am J Transplant. 2019 Mar 13;19(7):1917–1929. doi: 10.1111/ajt.15309

Figure 4. Extracellular mitochondria cause human ECs to upregulate vascular adhesion molecules.

Figure 4.

Primary human aortic ECs (HAECs) were treated with mitochondria purified from HeLa cells and analyzed for the upregulation of adhesion molecules CD54, CD106, and CD62E by flow cytometry. A) Dose response of the upregulation of adhesion molecules by HAECs following treatment with purified mitochondria (12 hours). B) Time response of EC upregulation of adhesion molecules following treatment with increasing exposure time of purified mitochondria (100 μg/mL). C-E) HAECs treated with mitochondria (100 μg/mL for 12 hr) demonstrate increased secretion of IL-6, IL-8, and MCP-1. HAECs without treatment or treated VS were used as controls. Comparisons were performed using a Kruskal-Wallis with Dunn’s multiple comparison test. *A p-value <0.05 was considered significant. In A & B, experimental groups were compared to no treatment.