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A Biotin/Neutravidin-Rhodamine (NA-Rh) assay reveals that Neutravidin (NA) penetration is limited to a radial distance that is dictated by photopolymerization conditions. Elucidating the governing reaction-diffusion behavior allows the network architecture to be defined, dictating local hydrogel mechanical properties and biomolecular diffusion for applications such as drug release or bioassays. rmax: radial position of the maximum florescence intensity.
