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. Author manuscript; available in PMC: 2019 Jun 24.
Published in final edited form as: Cell Rep. 2019 May 14;27(7):1979–1990.e7. doi: 10.1016/j.celrep.2019.04.084

Figure 1. Loss of PIP4K Increases PIP3 Levels.

Figure 1

(A and B) Representative confocal images with the distribution and quantification of PIP3 levels using GFP::PH-GRP1 probe in (Ai and Aii) larval salivary glands and (Bi and Bii) larval fat body. Scale bars: 50 μm in salivary gland images; 10 μm in fat body images.

(C) Total PIP3 levels using LCMS in whole larval lipid extracts.

(D) Confocal z-projections depicting PIP3 levels in (i and ii) insulin-stimulated (10 μM insulin, 10 min) salivary glands.

(E) Fat body lobes stimulated with 0.1, 1, and 10 μM insulin after 2-h starvation. Scale bars: 50 μm. (iii) Mean fold change in response to insulin (from data in ii).

(F) Total PIP3 levels using LCMS in insulin-stimulated whole larval lipid extracts.

Boxplots with whiskers at minimum and maximum values, a line at the median, and scatterplots with mean ± SD are shown. Numbers in parentheses below the plots indicate the number of biological replicates. Statistical tests: (A, B, and D) Mann-Whitney test and (C, E, and F) Student’s unpaired t test. *p value < 0.05. See also Figures S1 and S2.