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. Author manuscript; available in PMC: 2019 Jun 24.
Published in final edited form as: Cell Rep. 2019 May 14;27(7):1979–1990.e7. doi: 10.1016/j.celrep.2019.04.084

Figure 2. dPIP4K Cell-Autonomously Controls PIP3 Levels.

Figure 2

PIP3 levels in salivary glands.

(A) Mutant and wild-type dPIP4K rescue.

(B) Control, mutant, and kinase-dead dPIP4K rescue.

(C) (i) Immunoblot (from wandering third instar larvae) showing salivary gland-specific depletion of dPIP4K (dPIP4KRNAi). (ii) PIP3 levels in control and dPIP4KRNAi salivary glands.

(D) (i) Immunoblot for dPIP4K knockdown in S2R+ cells with two different dsRNAs. (ii) Total PIP3 using LCMS in whole cell lipid extracts of S2R+ cells treated with indicated dsRNAs (data pooled from two experiments, a total of four biological replicates). Scatter plots with mean ± SD. Statistical test: one-way ANOVA with post hoc Tukey’s multiple pairwise comparison. *p value < 0.05; **p value <0.01.

(E) Western blots for (i) pAKTT342 and (ii) pS6KT398 levels in control and dPIP4K knockdown cells.

See also Figure S2.