Figure 5.

The ILS1 conditional mutant constructed a unique biofilm regulating the classical biofilm-related genes and key elements. (A) Principal component analysis (PCA) and heatmap of RNA-seq data of the WT planktonic cells, the WT biofilm, and the ILS1 biofilm. (B) Heatmap of differential expression of biofilm-related genes³ in the RPMI biofilm (WT biofilm vs. WT planktonic cells) and the ILS1 conditional biofilm (ILS1 biofilm vs. WT biofilm). (C) Heatmap of up-regulated (>1.5 fold- change, adjusted p-value < 0.01) and down-regulated (<−1.5 fold- change, adjusted p-value < 0.01) genes in the ILS1 conditional biofilm compared with the WT biofilm (RPMI biofilm). The single down-regulated gene in both biofilms, ILS1, is pointed out by the arrow.