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Locus multiplexing and amplification conditions
| PCR1 | PCR2 | PCR3 | PCR4 | PCR5 | PCR6 | |
|---|---|---|---|---|---|---|
| Annealing temp. °C | 53 | 57 | 59 | 54 | 56 | 58 |
| Loci | WSV8 | WSV4 | WSV3 | WSV1 | WSV24 | WSV2 |
| 2 pmol of each forward and reverse primer for each locus per reaction | WSV12 | WSV7 | WSV16 | WSV6 | WSV31 | WSV17 |
| WSV13 | WSV21 | WSV10 | WSV20 | |||
| WSV15 | WSV36 | WSV11 | WSV22 | |||
| WSV32 | WSV14 | WSV25 | ||||
| WSV18 | WSV26 | |||||
| WSV19 | WSV27 | |||||
| WSV23 | WSV28 | |||||
| WSV29 | WSV33 | |||||
| WSV30 | WSV34 | |||||
| WSV35 | ||||||
| Q-solution (1.5 µL per reaction) | Yes | No | No | No | No | No |
