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. 2019 Jun 24;5:107. doi: 10.1038/s41420-019-0187-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Lysate of mature blood stages of malaria parasites demonstrated PA dependent phosphatase activity. Lysate of mature blood stages of parasite release Pi in the presence of PA which is inhibited by propranolol (125uM) indicating activity of PfPAP2. **P < 0.01, (n = 3). b Lysate of mature blood stages of malaria parasites accumulates PA in the presence of propranolol (125 μM). **P < 0.01, (n = 2). c (i) PA accumulation in parasite lysate in the presence of propranolol (125 μM) was monitored by LC-MS which demonstrated higher intensity peak of 647.29 m/z that corresponds to PA. (ii) Quantification of LC-MS peak intensity establishes accumulation of PA in response to propranolol