Fig. 2.

Dose-response of the mPF-PuroR gene circuit. a Network schematic of the mPF-PuroR gene circuit induced by Doxycycline (Dox), which expresses the reverse tetracycline transactivator (rtTA) regulator, the Puromycin resistance gene (PuroR) and EGFP separated by the self-cleaving 2A elements. The rtTA regulator activates its own expression upon binding Dox (red dashed line). b Normalized mean expression under varying levels of Doxycycline induction. c Gene expression noise amplitude (normalized coefficient of variation, CV) in response to Doxycycline induction. Error bars denote the standard error of the mean. There is an x-axis break (//) between 50 and 500 ng/mL Doxycycline. All samples were measured in triplicate (n = 3). d Single-cell gene expression distributions of mPF-PuroR cells with broad peaks at intermediate levels of Doxycycline. The legend displays Doxycycline concentrations per distribution. Distributions are from representative replicates. Source data are provided as a Source Data file