Fig. 3.

Dose-response of the mNF-PuroR gene circuit. a The mNF-PuroR gene circuit controls the expression of a Puromycin resistance gene and the EGFP reporter gene through inducible negative auto-regulation (blue dashed line) of a humanized tetracycline repressor (hTetR) gene. The 2A peptides self-cleave after translation. b Normalized mean expression of mNF-PuroR cells under varying levels of Doxycycline (Dox). c Gene expression noise of mNF-PuroR cells in response to Doxycycline. Error bars denote the standard error of the mean. There is an x-axis break (//) between 50 and 500 ng/mL Dox. All samples were measured in triplicate (n = 3). d Single-cell gene expression distributions of the mNF-PuroR circuit. The legend indicates Doxycycline concentrations for each distribution. Distributions are from representative replicates. Source data are provided as a Source Data file