Fig. 7.

Temporary drug removal suggests PuroR-dependent resistance mechanisms. a Schematic for the drug removal and retreatment experiment. Doxycycline (Dox) was removed or maintained simultaneously with drug removal. b Mean expression for mNF-PuroR during temporary removal of 35 μg/mL Puromycin and after final retreatment (FT). c, d Mean expression for mPF-PuroR after removal of (c) 35 and (d) 50 μg/mL Puromycin and after final retreatment (FT). e–g Growth curves (top) and adaptation times (bottom) during re-treatment for (e) mNF-PuroR cells under 35 μg/mL, and mPF-PuroR cells under (f) 35 and (g) 50 μg/mL Puromycin. The solid and dashed black lines in panels b–d indicate uninduced and induced baseline mean expression levels, respectively, prior to treatment with  Puromycin. The black growth curves are averaged untreated cell counts from Fig. 5c. Source data are provided as a Source Data file