Fig. 3. CLRN1-AS1 acted as a sponge of miR-217 in pituitary prolactinoma cell lines.

a, b Localization of CLRN1-AS1 in pituitary prolactinoma cell lines. c The binding of CLRN1-AS1 to RISC was detected by Ago2-RIP assay. d Eleven miRNAs that potentially bind with CLRN1-AS1 were subjected to luciferase reporter assay. e, f Expression level of four miRNAs in response to the overexpression or knockdown of CLRN1-AS1. g miR-217 expression in pituitary prolactinoma samples and the expression correlation between miR-217 and CLRN1-AS1. h The binding sequence between CLRN1-AS1-WT/MUT and miR-217 was used for luciferase reporter assay. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus control group, indicated data are statistically significant