FIGURE 3.

Anti-Xanthomonas activity of L. brunescens OH23 and its corresponding rpf mutants with or without LbDSF addition. (A) Supernatants from L. brunescens OH23 or rpf mutant cultures with or without exogenous LbDSF supplementation were tested for their antimicrobial activity against X. oryzae pv. oryzae PXO99^A. (B) The analysis of the images of X. oryzae pv. oryzae PXO99^A growth inhibition zones shown in A. (C) Representative images of IR24 leaves infected with X. oryzae pv. oryzae PXO99^A and treated with test supernatants. CK is the control, which was treated with water every 24 h. Images were taken 7 days post-inoculation (dpi). (D) Analysis of the lesion lengths on IR24 rice leaves caused by X. oryzae pv. oryzae PXO99^A infection with or without treatment with test supernatants, as shown in C. Different numbers of star (^∗) above the bars indicate a significant difference between the wild-type strain OH23 and mutant strains (ns: not sigificant, ^∗∗∗P < 0.001; ^∗∗∗∗P < 0.001, t-test).