Table 2.
Primers used in this study.
| Primer name | Sequence (5′ → 3′) | Amplicon size (bp) | Usage |
|---|---|---|---|
| rpfF-1 | CGAATTCCTGCAGCCCGGGGGATCCGCTGCTGCCATCGCGCAAGC | 535 | rpfF deletion |
| rpfF-2 | GGCATCAGGCCGACATGCGAGTGGAAGTAGAG | ||
| rpfF-3 | TCGCATGTCGGCCTGATGCCGATGATCACGGATCCGGACC | 535 | |
| rpfF-4 | CTGCCGCGGCAGCGGCCGCTCTAGATCGCATCGATCGCTGC | ||
| rpfG-1 | CGAATTCCTGCAGCCCGGGGGATCCACCGGCCAGCAGACCCCAGGA | 535 | rpfG deletion |
| rpfG-2 | GTGGGGCAATGGGCTGGAGTGAACGCGCTGCGCGCCGCCTAC | ||
| rpfG-3 | ACTCCAGCCCATTGCCCCACATGCGGATAC | 535 | |
| rpfG-4 | CTGCCGCGGCAGCGGCCGCTCTAGACCTCGGTGTCGGCATCGACCG | ||
| rpfC-1 | TCCTGCAGCCCGGGGGATCCTCGAAGGCGACGCGTTGGGTG | 528 | rpfC deletion |
| rpfC-2 | TGAACGCGTGATCATCGGCATCAGGCCGTGGCGGCATCG | ||
| rpfC-3 | GATGATCACGCGTTCACTCCAGCCCCGGCCGTTG | 528 | |
| rpfC-4 | GCGGCAGCGGCCGCTCTAGAATGCCCGAGGACGAGGTCCG | ||
| M13-F | TGTAAAACGACGGCCAGT | Confirmation of vector construction | |
| M13-R | CAGGAAACAGCTATGACC | ||
| RT-pilA1-F | AAGCCGAACGTCCAGATATC | 127 | pilA1 expression detection |
| RT-pilA1-R | GGCTGGAATTCGAGGAATAC | ||
| RT-recA-F | GTCACCGAAATCCTCTATGG | 164 | |
| RT-recA-R | GGGTTGTCCTTCATGTACTG |