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. 2019 Jun 24;10(7):496. doi: 10.1038/s41419-019-1731-x

Fig. 1. Preso contributes to neurotoxicity after traumatic neuronal injury.

Fig. 1

Traumatic neuronal injury (TNI) was induced in mouse neuronal cultures for 24 h. The representative images of neuronal culture at 12 h after TNI were obtained from light microscopy and PI/Hoechst staining (a). Scale bar = 100 μm. The protein expression of Preso was analyzed by western blot (b). The data are presented as the mean ± SEM from five experiments. After transfection of LV-shCon and LV-shPreso and TNI, the protein expression of Preso was analyzed by western blot (c), the cell death rate was assessed by PI/Hoechst staining (d), and the cytotoxicity was determined by an LDH assay (e). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. control and #p < 0.05 vs. LV-shCon. After transfection of LV-Con and LV-Preso and TNI, the protein expression of Preso was analyzed by western blot (f), the cell death rate was assessed by PI/Hoechst staining (g), and the cytotoxicity was determined by an LDH assay (h). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. control and #p < 0.05 vs. LV-Con