Skip to main content
. 2019 Jun 24;10(7):496. doi: 10.1038/s41419-019-1731-x

Fig. 2. Preso regulates NMDAR-mediated excitotoxicity.

Fig. 2

Mice cortical neuronal cultures were transfected with LV-Con and LV-Preso. After transfection, the neuronal cultures were pretreated with DL-AP5 (100 μM) and MK-801 (100 μM). Cytotoxicity and the cell death rate were analyzed in neuronal cultures after traumatic neuronal injury (a, b). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-Con and #p < 0.05 vs. Vehicle. Mouse cortical neuronal cultures were transfected with LV-shCon and LV-shPreso. After transfection, the neuronal cultures were treated with NMDA (100 μM). Cytotoxicity and the cell death rate were analyzed in neuronal cultures after NMDA treatment (c, d). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-shCon and #p < 0.05 vs. Con. After transfection of LV-shCon and LV-shPreso and TNI, the expression of NR1, NR2A, and NR2B was analyzed by western blot (E-H). The data are presented as the mean ± SEM from five experiments