Fig. 3. Regulation of NMDAR-related Ca²⁺/NO responses by Preso.

Mouse cortical neuronal cultures were transfected with different lentiviruses. After transfection, the intracellular Ca²⁺ concentrations and NO production were analyzed after traumatic neuronal injury for 12 h (a-c). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-shCon and ^#p < 0.05 vs. LV-Con. Scale bar = 50 μm. Mouse cortical neuronal cultures were transfected with LV-Con and LV-Preso. After transfection, the neuronal cultures were pretreated with DL-AP5 (100 μM) and MK-801 (100 μM). The intracellular Ca²⁺ concentrations and NO production were analyzed at 12 h after TNI (D-E). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-Con and ^#p < 0.05 vs. Vehicle. After transfection, the neuronal cultures were pretreated with BAPTA-AM (10 μM) and ARL 17447 (20 μM). Cytotoxicity and the cell death rate were analyzed in neuronal cultures at 12 h after TNI (E-F). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-Con and ^#p < 0.05 vs. Vehicle