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. 2019 Jun 24;10(7):496. doi: 10.1038/s41419-019-1731-x

Fig. 5. Interaction between Preso and PSD-95 regulates the NMDAR-induced NO response and excitotoxicity.

Fig. 5

Mouse cortical neuronal cultures were transfected with LV-shCon and LV-shPreso. After traumatic neuronal injury, the expression of PSD-95 and nNOS was analyzed by western blot (a-c). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-shCon. Mouse cortical neuronal cultures were transfected with different lentiviruses. After TNI, immunoprecipitants were obtained by PSD-95 immunoprecipitation. The expression of NR2B and nNOS was analyzed by western blot (d-f). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-shCon and #p < 0.05 vs. LV-Con. The cytotoxicity, cell death rate, and NO production in neuronal cultures were analyzed at 12 h after TNI (g-i). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-Con and #p < 0.05 vs. LV-Preso