Fig. 6. Involvement of CDK5 in the Preso regulation of the NMDAR-related Ca²⁺ response.

Mouse cortical neuronal cultures were transfected with different lentiviruses. CDK5 activity was analyzed after traumatic neuronal injury (a). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-shCon and ^#p < 0.05 vs. LV-Con. After transfection with LV-Preso, the neuronal cultures were pretreated with purvalanol B (50 μM). The intracellular Ca²⁺ concentrations were analyzed at 12 h after TNI (b). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. Vehicle. Mouse cortical neuronal cultures were transfected with LV-Con and LV-CDK5. The intracellular Ca²⁺ concentrations were analyzed at 12 h after TNI (c). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-Con. After transfection, the neuronal cultures were pretreated with DL-AP5 (100 μM). The intracellular Ca²⁺ concentrations were analyzed at 12 h after TNI (d). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. Vehicle