Fig. 7. Preso regulates NR2B phosphorylation by modulating CDK5 activity.

Mouse cortical neuronal cultures were pretreated with purvalanol B (50 μM). After traumatic neuronal injury, the phosphorylation of NR2B at Ser1284 was analyzed by western blot (a). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. Vehicle. Mouse cortical neuronal cultures were transfected with different lentiviruses. NR2B phosphorylation was analyzed by western blot at 12 h after TNI (b). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-shCon and ^#p < 0.05 vs. LV-Con. After transfection with LV-Preso ΔD, the NR2B phosphorylation, CDK5 activity, and intracellular Ca²⁺ concentration were analyzed at 12 h after TNI (c-e). The data are presented as the mean ± SEM from five experiments. *p < 0.05 vs. LV-Con and ^#p < 0.05 vs. LV-Preso