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. 2019 Jun 24;9:9096. doi: 10.1038/s41598-019-43958-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of artifacts on the percentage non-perfusion area (PNPA) metric of a 12 mm × 12 mm OCTA image of the retinal vasculature of an eye with mild NPDR from a 68 year-old. (A) En face OCTA image showing many regions of low OCTA signal; from this en face OCTA image alone, it is unclear if all regions of low OCTA signal correspond to regions of actual low/no blood flow, or whether some are false positives caused by low-OCT-signal artifacts. (B) The uncorrected identification of non-perfusion areas (teal). (C) The corrected identification of non-perfusion areas, where regions of low-OCT-signal artifacts have been manually excluded (see Fig. 4). The PNPAs, given in the top right corner of panels (B,C), change from 22% (uncorrected) to 13% (corrected).