Table 2.
IC50 values (± the standard error determined in GraFit 5.0) for P. aeruginosa PAO1 PBPs for the single antibiotic treatments (ATM, CAZ or MEM) and the dual combination treatments (MEM + ATM and MEM + CAZ).
| Beta-lactam treatment | IC50 (mg/L) | ||||||
|---|---|---|---|---|---|---|---|
| PBP1a | PBP1b | PBP2 | PBP3a | PBP4 | PBP5 | PBP7 | |
| ATM | 0.904 ± 0.283 | 0.816 ± 0.543 | 4.56 ± 3.746 | >1000 | 5.449 ± 2.035 | >1000 | >1000 |
| CAZ | 0.469 ± 0.159 | 4.48 ± 4.33 | 1.329 ± 1.134 | >1000 | 8.022 ± 2.19 | >1000 | >1000 |
| MEM | 0.105 ± 0.053 | 0.037 ± 0.009 | 0.744 ± 1.251 | 6.034 ± 4.878 | 0.003 ± 0.0002 | 0.3697 ± 0.271 | >1000 |
| ATM + MEM | 0.158 ± 0.068 | 0.067 ± 0.022 | 15.884 ± 19.289 | >1000 | 0.004 ± 0.0011 | 5.498 ± 4.649 | >1000 |
| CAZ + MEM | 0.631 ± 0.326 | 0.082 ± 0.042 | 17.987 ± 26.859 | >1000 | 0.004 ± 0.0005 | 6.724 ± 2.874 | >1000 |
Underlining shows lowest IC50 for each β-lactam treatment − indicating selectivity.