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. 2019 Jun 24;9:9116. doi: 10.1038/s41598-019-45547-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Absence of Tau does not alter NMDA receptor levels in synaptosome-enriched hippocampal fractions. Western blot (WB) analysis of the levels of Tau, PSD95, GIPC1 and GluN2B receptor subunits of NMDA receptors in synaptosome-enriched fractions obtained from the hippocampi of WT and Tau^−/− mice. (A) Tau is not present in synaptosome-enriched fractions obtained from the hippocampi of Tau^−/− mice, and this absence does not affect the levels of PSD95 or those of GluN2B subunits of NMDA receptors detected with an antibody directed against its C-terminus. Quantifications are shown on the right. (B) The levels of GIPC1 are not altered in Tau^−/− synaptosome-enriched fractions as compared to WT ones. Quantification is shown on the right. (C) The levels of GluN2B subunits of NMDA receptors detected with an antibody directed against its N-terminus are not altered in synaptosome-enriched fractions of Tau^−/− mice as compared to WT ones. Quantification is shown on the right. (D) The levels of GluN2B subunits of NMDA receptors phosphorylated at Y1472 (a phosphorylation occurring mainly at synaptic sites) tend to decrease in synaptosome-enriched fractions of Tau^−/− mice as compared to WT ones. Quantification is shown on the right. (E) The levels of GluN2B subunits of NMDA receptors phosphorylated at Y1336 (a phosphorylation occurring mainly at extrasynaptic sites) are not significantly altered in synaptosome-enriched fractions of Tau^−/− mice as compared to WT ones. Quantification is shown on the right. Graphs show mean ± SEM. N WT = 5 mice; N Tau^−/− = 4 mice. The levels of pGluN2B(Y1472) and pGluN2B(Y1336) were calculated related to the levels of GluN2B N-ter. Actin was used as loading control. Blots were cropped to improve the clarity of the presentation. Full-length blots are presented in Supplementary Fig. 5.