Fig. 6.
Stepwise construction of polyproteins with a rationally-controlled sequence. a The schematic of the stepwise, ligation and cleavage procedure to construct sequence-controlled polyprotein on the glass surface. b Representative curves with maximum unfolding peaks from the unfolding experiments of homo-polyprotein (Ub)5, top graph, (Rd)5, middle graph, and (Ub-Rd)3, bottom graph, are shown, which were detected during each polymerization step from one to five/six for the same sample. c The statistical analysis of the number of the curve with specific unfolding peaks, corresponding to the left curves