Fig. 4.

Large cavernomas are mosaic. a Representative images of chronic Cdh5(PAC)-Cre-ER^T2/Ccm3^f/f mice co-stained for KLF4 (green) and either ERG1 or COLLAGEN IV (red). b Quantification of the percentage of KLF4-positive endothelial cells lining the cavernomas of chronic P8, P14 and P30 mice. Data are means ± SE; p < 0.001 among groups (one-way analysis of variance); *p < 0.01 (Tukey’s post hoc test); n = 3 mice in each group. Source data are provided as a Source Data file. c–h Representative images of brain sections co-stained for PECAM1 (red) and mesenchymal markers (green), showing small lesions from P8 mice and large cavernomas from P14 mice. White arrows, endothelial cells positive for mesenchymal markers within the lesions; yellow arrows, endothelial cells negative for mesenchymal markers in normal vessels; blue arrows, endothelial cells negative for mesenchymal markers within the lesions. Scale bars, 100 μm