Fig. 6.

Ccm3^−/− cells form cavernomas and recruit Ccm3^+/+ cells in wild-type animals. a Endothelial cells were isolated from acute Cdh5(PAC)-Cre-ER^T2/Ccm3^fl/fl/R26R-Confetti at P7 and re-injected into the brains of adult wild-type mice. b Brain section showing the formation of aberrant vessels that comprise both Confetti-positive endothelial cells, which are Ccm3^−/− coming from the donor, as well as non-labelled Ccm3^+/+ endothelial cells coming from the host. c In the same way, endothelial cells were isolated from acute Cdh5(PAC)-Cre-ER^T2/Ccm3^fl/fl at P7 and re-injected into the brains of adult Cdh5(PAC)-Cre-ER^T2/R26R-Confetti mice treated with 2 mg of Tamoxifen 1 week before injection. d Brain section showing the formation of aberrant vessels, which comprise also Ccm3^+/+ Confetti-positive endothelial cells coming from the  host. e Endothelial cells were isolated from acute Cdh5(PAC)-Cre-ER^T2/Ccm3^fl/fl at P7 and engrafted in a subcutaneous Matrigel plug into adult Cdh5(PAC)-Cre-ER^T2/R26R-Confetti mice. f Section of the plug showing the formation of aberrant vessels, which comprise both non-labelled Ccm3^−/− endothelial cells coming from the donor as well as Confetti-positive endothelial cells, thus Ccm3^+/+ coming from the host. White arrows, endothelial cells positive for Confetti; yellow arrows, endothelial cells negative for Confetti. Scale bars, 100 μm