Fig. 3.

Chemogenetic silencing of ovBNST PKC-δ neurons increases feeding. a, b Food intake was increased when ovBNST PKC-δ neurons were chemogenetically silenced in fed (a) and fasted (b) animals. Two-way ANOVA with post-hoc Bonferroni’s t test, F_1,51 = 9.49, n = 8, 12, 16, and 16 animals for mCherry-Saline, mCherry-CNO, hM4Di-saline, and hM4Di-CNO, respectively. c–f The latency to eat (c), bout duration (d), bout number (e), and total time that the animals spent in feeding (f). Unpaired t-test, t(30) = 0.48 (c), t(30) = 3.35 (d), t(30) = 0.71 (e), t(30) = 3.24 (f, total feeding time). n = 16 in each group. Data are mean ± s.e.m. **p < 0.01, ***p < 0.001. Source data are provided as a separate file