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. 2019 Jun 24;9:9189. doi: 10.1038/s41598-019-45573-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TGCT specific miRNA expression in TGCT cells after cisplatin treatment. The expression of ten miRNAs was analysed by qPCR in both 833 K and NT2-D1 cells after 24-hour incubation with cisplatin (6 μM). Both in 833 K (a) and NT2-D1 cells (b), the expressions of miR-302a-3p, miR-302b-3p and miR-302c-3p were significantly downregulated while the downregulation of miR-302d-3p was only significant in NT2-D1 cells. The miR-302a-5p, miR-200c-3p and miR-367-3p showed no significant changes, and the expression of the miR-371-373 cluster was not detected in either cell line. Relative fold change of miRNA expression was determined using the equation RQ = 2^−ΔΔCT. t-test: Control vs Cisplatin (6 μM), mean ± SD (calculated from three independent experiments), statistical significance p < 0.05. The designation on the x-axis indicates the miRNA.