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. 2019 Jun 24;9:9189. doi: 10.1038/s41598-019-45573-6

Figure 3.

Figure 3

Inhibition of miR302a-3p, miR-302b-3p, and miR-302c-3p in TGCT cells. miRNA inhibitor-mediated transient transfection reduced the expression levels of all three miRNAs by more than 80% in 833 K (a) and NT2-D1 cells (b), as analysed by qPCR. A non-targeting, miRNA inhibitor negative control with the same chemical modifications was used as a vehicle control. Relative fold change of expression was determined using the equation RQ = 2−ΔΔCT. The designation on the x-axis indicates the miRNA inhibitor.