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. 2019 Jun 24;9:9183. doi: 10.1038/s41598-019-45137-8

Figure 2.

Figure 2

Liposome uptake and organization of the tight junction protein ZO1 in BMEC monolayers after liposomes incubation. (A) Photomicrographs of BMEC monolayers subjected to incubation of Lipo and gH625-Lipo loaded at 5 µM PACAP-Rho for 60 min on live cells (Scale bars, 10 µm). Nuclei were stained with Höechst. (B) Photomicrographs of BMEC monolayers subjected to incubation of Lipo and gH625-Lipo loaded at 5 µM PACAP-Rho for 60 min on live cells. Cells were fixed, permeabilized and stained with an anti-ZO1 antibody (Scale bars, 10 µm). Nuclei were stained with Höechst. Note that the photomicrographs show no significant difference between the Lipo and gH625-Lipo groups compared to control.