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. 2019 Jun 24;9:9183. doi: 10.1038/s41598-019-45137-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of uptake by and transport across BMEC monolayers of liposomes at different times. Quantification of Lipo and gH625-Lipo loaded at 5 µM PACAP-Rho in different compartments of the in vitro BBB model after incubation in the luminal compartment in contact with BMEC monolayers for 30, 60 and 120 min at 37 °C. (A) Amounts of PACAP-Rho in the luminal compartment; (B) PACAP-Rho in the BMEC lysate; (C) PACAP-Rho in the abluminal compartment. Values represent means ± SD of 5 independent experiments (*P < 0.05; **P < 0.005; ***P < 0.0005).