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. 2019 Jun 24;9:9100. doi: 10.1038/s41598-019-45298-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of transgene expression in H4 cells. Overview of αSyn constructs and induction of their overexpression. (A) αSyn-140 (S). (B) αSyn-140 coupled to the fluorescence protein Venus (SV). (C) αSyn-140 coupled to the N-terminal (V1) or C-terminal (V2) part of Venus. Aggregation of αSyn results in fluorescence due to complementation of the Venus fragments. (D) Overexpression in the Cre_ER^T2-loxP system (CE^T2) is induced by 4-OH-tamoxifen. (E) Overexpression in the GAL4_EcR-UAS system (GE) is induced by tebufenozide.