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. 2019 Jun 24;9:9100. doi: 10.1038/s41598-019-45298-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression characteristics of H4_GE cells – cellular fluorescence. H4 cell lines were incubated for up to 8 days with DMSO or with different tebufenozide concentrations ranging from 100 pM to 100 µM and imaged using the Opera^® system. Quantification was performed using the Acapella^® software. (A) Quantification of the mean cellular Venus fluorescence intensity of H4 cells upon incubation with DMSO or different concentrations of tebufenozide (color-coded). (B) Quantification of the fraction of Venus-positive H4 cells. (C) Quantification of the mean cellular Venus fluorescence intensity of H4 cells in dependence of the tebufenozide concentration using the dataset from A) at DPI 5. (D) Quantification of the number of cells per image field. Data points show mean of 3–6 independent experiments; error bars show SEM.