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. 2019 Jun 24;10(7):492. doi: 10.1038/s41419-019-1738-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a ID2 was silenced in hAMSCs using two independent siRNAs (si-ID2-2 and si-ID2-3). The knockdown efficiency was verified by qRT-PCR compared with the negative control (NC). b qRT-PCR analysis detected DE marker genes (SOX17, FOXA2, and CXCR4) in ID2-knockdown hAMSCs and control hAMSCs on day 5 after DE induction. c Western blot detected the expression of ID2, SOX17, and FOXA2 in ID2-knockdown hAMSCs and control hAMSCs on day 5 after DE induction. d Flow cytometry analysis of the FOXA2⁺/SOX17⁺ subpopulation in si-ID2 hAMSC or si-NC hAMSCs on day 5 after DE induction. e hAMSCs were transduced with lentivirus overexpressing ID2 (Lenti-ID2) or empty vectors (Lenti-NC). The efficiency of ectopic expression was verified by qRT-PCR. f qRT-PCR analysis detected DE marker genes (SOX17, FOXA2, and CXCR4) in Lenti-ID2 hAMSCs or Lenti-NC hAMSCs on day 5 after DE induction. g Western blot detected the expression of ID2, SOX17, and FOXA2 in Lenti-ID2 hAMSCs or Lenti-NC hAMSCs on day 5 after DE induction. h Flow cytometry analysis of the FOXA2⁺/SOX17⁺ subpopulation in lenti-ID2 hAMSCs or Lenti-NC hAMSCs on day 5 after DE induction. Data are shown as the means ± S.D. (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001