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. 2019 Jun 24;9:9081. doi: 10.1038/s41598-019-45282-0

Figure 3.

Figure 3

Continuous sedimentation coefficient distribution of m-NAD(P)-ME with different nucleotide ligands. The enzyme concentrations were 1.6, 4.7, and 14.2 μM in the phosphate buffer with L-malate (20 mM), pyruvate (8 mM), MgCl2 (2 mM) or fumarate (0.2 mM). (ah) with NAD+ (0.4 mM); (ip) with ATP (0.5 mM); and (qx) ADP (with 1 mM).