. 2019 Jun 24;9:9081. doi: 10.1038/s41598-019-45282-0

Table 4.

Common features of m-NAD(P)-ME and PFK-1.

Feature	m-NAD(P)-ME	PFK-1
Subcellular Location	Mitochondria	Cytosol
Quaternary Structure	Tetramer (most active) Dimer (less active)	Tetramer (most active) Dimer (less active)
Allosteric Nucleotide-binding Site	One site Exosite at the tetramer interface; can bind to NAD⁺, ATP or ADP^{22–24,27,28} NAD⁺ binding: Enhances the formation of a tetrameric structure ATP binding: Promotes the dissociation of tetramers into dimers	Two sites Activating nucleotide-binding site can bind to ADP or AMP. Inhibitory nucleotide-binding site can bind to ATP or ADP. ATP binding: Promotes the dissociation of tetramers into dimers^42,43
Nucleotide Substrate	NAD⁺	ATP (<1 mM)
Nucleotide Inhibitor	ATP (IC₅₀ = 0.2 mM) ADP (IC₅₀ = 3.7 mM) tetramers disassociate into dimers	ATP (>1 mM) ADP (at mM) tetramers disassociate into dimers
Nucleotide Activator	NAD⁺	ADP (at μM)