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. 2019 Jun 24;9:9134. doi: 10.1038/s41598-019-45600-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Blood parameters are altered by the HF/HS diet. Plasma glucose (a), cholesterol (b) and triglyceride levels (c) were measured in mice fed either the chow diet (Std) or the high-fat/high sucrose diet (HF/HS), after 0, 3 and 6 months of supplementation. Data are presented as the means ± s.e.m. and were analysed using ANOVA; n = 20 (Std) and n = 20 (HF/HS) per time point. ***p < 0.001. The size distribution of the plasma HDL lipoproteins was assessed by using electrophoresis on Spiragel™ 1.5–25% in mice fed either the chow diet (Std) or the high-fat/high sucrose diet (HF/HS) after 90 (d) and 180 (e) days of supplementation. Data are presented as the means ± s.e.m. and were analysed using Student’s unpaired t-test; n = 20 (Std) and n = 19 (HF/HS) per time point. **p < 0.01 and ***p < 0.001. (f) Plasma MDA levels in mice fed either the chow diet (Std) or the high-fat/high sucrose diet (HF/HS) were measured after 180 days of supplementation. Data are presented as the means ± s.e.m. and were analysed Student’s unpaired t-test; n = 20 (Std) and n = 14 (HF/HS). ***p < 0.001. Plasma total fatty acid (g) and lipopolysaccharide (h) levels in mice fed either the chow diet (Std) or the high-fat/high sucrose diet (HF/HS) were assessed by LC-MS/MS after 180 days of supplementation. Data are presented as the means ± s.e.m. and were analysed by using the Mann-Whitney U-test; n = 20 (Std) and n = 20 (HF/HS). ***p < 0.001.