Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 24;9:9134. doi: 10.1038/s41598-019-45600-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mobilization of lipids in adipose tissues by caloric restriction. The levels of total fatty acids (a), HODEs (b) and HETEs (c) were measured in the eWAT from mice fed mice fed either the HF/HS or HF/HS + PRPE diet for 180 days by using LC-MS/MS. Data are presented as the means ± s.e.m. and were analysed using the Mann-Whitney U-test, n = 20 (HF/HS) and n = 20 (HF/HS + PRPE) for a) and multiple Student’s unpaired t-tests; n = 20 (Std) and n = 20 (HF/HS) for (b,c). (d) Lipopolysaccharide (LPS) levels in the eWAT of mice fed either the HF/HS or HF/HS + PRPE diet for 180 days. Data are presented as the means ± s.e.m. and were analysed using the Mann-Whitney U-test; n = 20 (HF/HS) and n = 20 (HF/HS + PRPE). *p < 0.05. (e) Caloric restriction (CR) protocol: mice were fed ad libitum for 180 days with either the HF/HS or HF/HS + PRPE diet, and then a 30% reduction in energy intake was performed for an additional 120 days. Changes in the body weight of mice in each group are shown. Data are presented as the means ± s.e.m. and were analysed by using one-way ANOVA, n = 20 (HF/HS) and n = 20 (HF/HS + PRPE). ***p < 0.001. (f) eWAT cholesterol and cholesterol oxide content was assessed by GC/MS in fasted mice fed the HF/HS or HF/HS + PRPE diet for 180 days (upper panel, before CR) and after 120 days of caloric restriction (lower panel, after CR). Data are presented as the means ± s.e.m. and were analysed by using the Mann-Whitney U-test; n = 20 (HF/HS) and n = 20 (HF/HS + PRPE) before and after CR. ***p < 0.001.