Fig. 1.

Ca²⁺-dependent Cl⁻ currents in human bestrophin1 (hBest1) gate mutants. a–c Representative current traces in the presence of 1.2 μM Ca²⁺, and population steady-state current density–voltage relationships in HEK293 cells individually expressing hBest1 wild-type (WT) (a), I205A (b), or 3A (c), with Cl⁻ in the external solution, in the absence (gray) or presence (black) of 1.2 μM Ca²⁺, n = 5–10 for each point. Insert, voltage protocol used to elicit currents. Scale bar, 300 pA, 150 ms. *P < 0.05 compared to cells in the absence of Ca²⁺, using two-tailed unpaired Student’s t test. d Bar chart showing the steady-state Cl⁻ current densities at 100 mV in the absence (gray) or presence (black) of Ca²⁺, n = 5–11 for each bar. *P < 0.05 compared to cells in the absence of Ca²⁺, using two-tailed unpaired Student’s t test. All error bars in this figure represent s.e.m.