Table 1.

Radii at critical residues in the ion-conducting pathway of bestrophins

KpBest		cBest1
Residue	Radius (Å)	Residue	Radius (Å)
I62	1.3	I76	1.1
I66	2.2	F80	1.4
F70	2.3	F84	2.3
I180	0.9	V205I	0.9
I180A	3.2	V205	1.3

Radius was calculated based on measuring the distances between five CH₃- (C delta on isoleucine or valine, a1–a5) or H- (benzene ring on phenylalanine, b1–b5):

For isoleucine or valine, radius = (a1 + a2 + a3 + a4 + a5)/(5 × 1.17) – 2.00 (van der Waals radius of CH₃-)

For phenylalanine, radius = (b1 + b2 + b3 + b4 + b5)/(5 × 1.17) – 1.20 (van der Waals radius of H-)