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. 2019 Jun 25;5:33. doi: 10.1038/s41421-019-0100-3

Fig. 1. Depletion of MLL2 expression or inhibition of its activity stimulates ciliogenesis.

Fig. 1

a Quantitative RT-PCR analysis of MLL2 mRNA expression in RPE-1 cells transfected with control or MLL2 siRNAs and serum-starved for 48 h. b Immunoblot analysis of H3K4me1 and β-actin levels in RPE-1 cells transfected with control (C) or MLL2 (M) siRNAs and serum-starved for 0, 12, 24, and 48 h. c–e Immunofluorescence images (c), percentage of ciliated cells (d, n = 200), and ciliary length (e, n = 80) for RPE-1 cells transfected with control or MLL2 siRNAs, serum-starved for 48 h, and stained with antibodies against γ-tubulin (red) and acetylated α-tubulin (green), and the DNA dye DAPI (blue). Scale bar, 10 µm. f–h Immunofluorescence images (f), percentage of ciliated cells (g, n = 200), and ciliary length (h, n = 50) for RPE-1 cells treated with the indicated concentrations of MTA, serum-starved for 48 h, and stained with antibodies against γ-tubulin (red) and acetylated α-tubulin (green), and DAPI (blue). Scale bar, 10 µm. i–k Immunofluorescence images (i), percentage of ciliated cells (j, n = 100), and ciliary length (k, n = 30) for RPE-1 cells transfected with the indicated siRNAs and plasmids, followed by serum starvation and staining with acetylated α-tubulin antibodies (red) and DAPI (blue). Scale bar, 10 µm. **P < 0.01; ***P < 0.001. Error bars indicate SEM