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. 2019 Jun 24;9:9099. doi: 10.1038/s41598-019-45284-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Topography induced differences in gene expression profiles. Microarray analyses of hMSCs after 7 days on topographically enhanced (S, M, and L) substrates compared to flat (N = 3). A) Venn diagram represents the number of DEGs which were unique for the topography conditions or which overlapped with the other condition(s). (B) Z-score scaled heatmap with DEGs, for each topography (S, M, and L), which were found in all three topographies. Underlined genes are involved in metabolic processes. (C) Proportional distribution representing the panther gene ontology classification analysis grouping the 34 DEGS to biological processes based on their gene ontology annotation. The list of DEGS linked to metabolic processes was specified further in a similar manner.