FIGURE 2.

Growth curves, 53D1 production (A,B), and chitinase activities (C,D) in S. coelicolor/pIJ86 (left panels) and S. coelicolor/pIJ86::53D1 (right panels), grown in YEME medium. In panels (A,B), wet weight (, dotted line), pH (, solid line), glucose consumption (•, dashed line), and 53D1 production determined by western blot analysis of cell-free culture broths (gray bars). In panels (C,D), chitinase activity of cell-free culture broths measured by fluorimetric activity assay on 4-MU-(GlcNAc)₂ as substrate (black bars).